Action on fibrinogen was electrophoretically and spectrophotometrically evaluated, fibrinolytic activity was measured both electrophoretically and by the fibrin plate assay, and the effect on blood coagulation was studied by conventional coagulation tests (PT and APPT). The aim of this work was to study the effect of Pm, Bb, and Bh on fibrin(ogen) and blood coagulation compared with stem bromelain (Bro). Given the close relationship between hemostasis and inflammation, it is attractive to investigate therapeutic agents capable of modulating both systems. hieronymi (Bh) have previously shown an anti-inflammatory effect on animal models. hieronymi (Bromeliaceae) in comparison with bromelain.Įrrasti, MarÃa E Prospitti, Anabela Viana, Carolina A Gonzalez, Mariana M Ramos, Márcio V Rotelli, Alejandra E Caffini, Néstor OĮxtracts rich in cysteine proteases obtained from fruits of Pseudananas macrodontes (Pm), Bromelia balansae (Bb), and B. The microwave assisted approach is a green, rapid and environmental friendly extraction technique for the production of bio-oil bearing crops.Įffects on fibrinogen, fibrin, and blood coagulation of proteolytic extracts from fruits of Pseudananas macrodontes, Bromelia balansae, and B. A rapid and environment friendly extraction technique is preferable to enhance bio-oil yield. The viscosity of bio-oil obtained by catalytic means increased from 20.5 to 37.8 cP. The pH of bio-oil increased from 3.5 to 4.3. Oil yield for non-catalytic extraction was 43.8% while with addition of seashell-based biocatalyst was 44.6%.
Oil yield for non-catalytic extraction was 43.8% while addition of seashell based biocatalyst was 44.6%. Bio-oil extraction from the pyrolysis of empty fruit bunch (EFB), a biomass waste from oil palm crop, was enhanced using a biocatalyst derived from seashell waste. Thus, this is a green technique of bio-oil extraction using only water as extractant. The MAE extraction system is rapid using only water as solvent which is a nonhazardous, environment-friendly technique compared to soxhlet extraction (SE) method using hexane as solvent. Bio-oil extracted using the microwave assisted extraction (MAE) system could enhance yield of bio-oil compared to soxhlet extraction. The biooil yield obtained from microwave assisted extraction system at 90 minutes was 36% while that from soxhlet extraction for 6 hours was 42%. Extraction was carried out at different extraction times 15 min, 30 min, 45 min, 60 min and 120 min to determine oil yield. Clean samples were heated in an oven at 110 ° C for 24 hours to remove moisture content and ground to obtain particle size smaller than 500μm. Jatropha seeds (Jatropha curcas) were used to extract bio-oil. The commonly practised organic solvent oil extraction technique could be replaced with a modified microwave extraction. The move towards a green bio-oil extraction technique is highlighted in this paper. These data may contribute to the advancement of biopharmaceutical applications for this species. These results show that EEBB has an anti-inflammatory potential without causing acute or subacute toxicity. A dose of 300 mg/kg of EEBB significantly reduced oedema formation, Cg-induced mechanical hypersensitivity and cold sensitivity, as well as leukocyte migration in the pleurisy model. Similarly, there were no statistically significant differences in the sperm parameters.
In the subacute toxicity test, the tested doses produced no significant changes in the haematological, biochemical or histopathological parameters of treated animals. A single oral dose of EEBB did not result in toxicity, demonstrating that the LD 50 of this extract was greater than 2000 mg/kg. The anti-inflammatory effect of EEBB was evaluated in vivo (30, 100, or 300 mg/kg) by carrageenan (Cg) induced-oedema and pleurisy in Swiss mice. balansae for the acute toxicity test and with 25, 100, or 400 mg/kg of EEBB for the subacute toxicity test. Male rats (Wistar) were gavaged with 2000 mg/kg of extract from the fruit of B.
This study assessed the anti-inflammatory activity of the ethanolic extract obtained from Bromelia balansae fruit (EEBB) as well as the toxicological potential of this extract after single and repeated exposure. Toxicological evaluation and anti-inflammatory potential of an ethanolic extract from Bromelia balansae (Bromeliaceae) fruit.ĭa Silva Balin, Paola Zanatta, Flavia Carina Jorge, Bárbara Campos Leitão, Maicon Kassuya, Roberto Mikio Cardoso, Claudia Andrea Lima Kassuya, Cândida Aparecida Leite Arena, Arielle Cristinaīromelia balansae is a relatively unexplored medicinal species that is used for nutritional purposes and in folk medicine to treat cough or wounds.